Evaluation of the chemical composition and antioxidant activity of Citrus limon essential oil and its application in margarine preservation

Louiza HIMED, Salah Merniz, Malika Barkat

Abstract


 

Essential oils are aromatic oil liquids obtained from various plant parts. The growing interest in the substitution of synthetic antioxidant agents by natural ones has fostered research on vegetable sources and the screening of plant materials in order to identify new compounds. The study aims to evaluate the use of essential oil extract by cold pression of Citrus limon (Lisbon variety) as a substitute of Tocoblend (mixture of α, β, γ and δ-tocopherol) used as antioxidant in margarine preservation. The major constituents of the essential oil extract Identified were Limonene (66.75%) followed by β-Pinene (13.92%) and γ-Terpinene (3.10%). In both DPPH scavenging and bleachability of β-carotene in linoleic acid system assays, the essential oil extract exhibited the highest activity compared to the Tocoblend. Tests conducted at pilot scale showed that the margarine elaborated with essential oil extract was more resistant to oxidation than the margarine reference with Tocoblend. In addition, the physicochemical properties were not modified.



Keywords


Citrus limon, essential oil, antioxidant activity, chemical composition, margarine

Full Text:

PDF

References


Sasaki, Y.F., Kawaguchi, S., Kamaya, A., Ohshita, M., Kabasawa, K., Iwama, K., Taniguchi, K., Tsuda, S., 2002. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat. Res. 519, 103–109.

Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., Vidal, N., 2006. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 97, 654–660.

Laufenberg, G., Kunz, B., & Nystroem, M. (2003). Transformation of vegetable waste into value added products: (A) the upgrading concept; (B) practical implementations. Bioresource Technology, 87(2), 167−198.

Chon, R., and Chon, A. L. (1997). In P. R. Ashurst (Ed.), Subproductos del Procesado de frutas. Acribia, Zaragoza (pp. 213−228).

Raeissi, S., Diaz, S., Espinosa, S., Peters, C. J., & Brignole, E. A. (2008). Ethane as an alternative solvent for supercritical extraction of orange peel oils. Journal of Supercritical Fluids, 45, 306−313.

Prabuseenivasan, S., Jayakumar, M., Ignacimuthu, S., 2006. In vitro antibacterial activity of some plant essential oils. BMC complement. Altern. Med. 6, 39.

Arctander, S. (1994). Perfume and flavor chemicals. Carol Stream, IL: Allured Publ.

Adams, R. P. (1995). Identification of essential oil components by gas chromatography/ mass spectroscopy. Carol Stream, IL: Allured Publ.

AFNOR (2000a). Monographie relative aux huiles essentielles, Paris.

Sahraoui, N., Abert Vian, M., Bornard, I., Boutekedjiret, C., Chemat, F. (2008). Improved microwave steam distillation apparatus for isolation of essential oils Comparison with conventional steam distillation. Journal of Chromatography A, 1210, 229–233.

Kartal N., Sokmen M. , Tepe B., Daferera D., Polissiou M., Sokmen A. (2007). Investigation of the antioxidant properties of Ferula orientalis L using has suitable extraction procedure. Food Chemistry.100: 584-589.

ISO International Standard. (1998a). Method 662. Animal and vegetable fats and oils. Determination of moisture content and volatile matter.

ISO International Standard. (1998b). Method 3960, Animal and vegetable fats and oils.

Determination of peroxide index.

ISO International Standard. (2002a). Method 6321, Animal and vegetable fats and oils.

Determination of melting point.

ISO International Standard. (1995). Method 8292 (F). Animal and vegetable fats and oils. Determination of the solids fat by the method of pulsed nuclear magnetic resonance (2nd ed.).

Farhoosh, R. (2007). The effect of operational parameters of the Rancimat method on the determination of the oxidative stability measures and shelflife prediction of soybean oil. Journal of American Oil Chemistry Society, 84, 205–209.

ISO International Standard. (2006). Method 6886. Animal and vegetable fats and oils.

Determination of the oxidation stability (accelerated oxidation test) (2nd ed.) pp. 1–14.

Himed L. and Barkat M. (2014). Élaboration d’une nouvelle margarine additionnée des huiles essentielles de Citrus limon. Oilseeds & fats Crops and Lipids, A102.

Mimica-Dukic, N., Bozin, B., Sokovic, M., Simin, N., 2004. Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. J. Agric. Food Chem. 54, 5313–5322.

Karleskind A., 1992; Manuel des Corps Gras.Ed Tech& Doc, Paris. Tome 1 et Tome 2. 1579p.

Bongers, P., & Almeida-Rivera, C. (2011). Dynamic modelling of the margarine production proces s. Computer Aided Chemical Engineering, 29, 1301–1305.

Hidalgo, F. J., Leon, M. M., & Zamora, R. (2006). Antioxidative activity of amino phospholipids and phospholipid/amino acid mixtures in edible oils as determined by the Rancimat method. Journal of Agricultural and Food Chemistry, 54, 5461–5467.

Please cite this Article as:

HIMED Louiza, MERNIZ Salah and BARKAT Malika, Evaluation of the chemical composition and antioxidant activity of Citrus limon essential oil and its application in margarine preservation, Algerian J. Nat. Products, 4:2 (2016) 292-298.

www.univ-bejaia.dz/ajnp

Online ISSN: 2353-0391

Editor in chief: Prof. Kamel BELHAMEL


Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 ALGERIAN JOURNAL OF NATURAL PRODUCTS

_________________________________________________________________________________________________________________________

 All open access articles published in AJNP are distributed under the terms of the  Creative Commons Attribution-  4.0 International License

The research works published in this journal are free to be accessed. They can be shared (copied and redistributed in any medium or format) and\or adapted (remixed, transformed, and built upon the material for any purpose, commercially and\or not commercially) under the following terms: attribution (appropriate credit must be given indicating original authors, research work name and publication name mentioning if changes were made) and without adding additional restrictions (without restricting others from doing anything the actual license permits). Authors retain the full copyright of their published research works and cannot revoke these freedoms as long as the license terms are followed.

 

 Flag Counter