Comparison of free radical scavenging capacities of methanol extract of two mint species by fixed point and kinetic methods

Shadia Mahmoud Sirry, Hind Ahmed Babiker


Mentha Longifolia (ML) and Mentha Puleguim (MP) are usually used as a flavor and medicinal plants in Saudi Arabia. The present study was carried out to compare the potential of methanol extract of fresh leaves of ML and MP as free radical (DPPH) scavenger by fixed point and kinetic methods. In a fixed point method, free radical scavenging capacity was expressed by effective concentration, EC50.  However, in kinetic method, capacity expressed by rate constant value. Some other antioxidants parameters like total flavonoids content and total phenolic content were also estimated. Flavonoid content was obtained as catechin equivalent and total phenolic content as gallic acid equivalent. The obtained results indicate that methanol extract of MP possess a high concentration of flavonoids (6.55± 0.42 mg/100 ml) and of phenolic (3.72 ±0.29 mg/100 ml) compared to that of ML flavonoids (3.22± 0.43 mg/100 ml) and of phenolic (1.91 ±0.06 mg/100 ml). 


chemistry /antioxidant/activity/evaluating natural products

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Fang, Yun-Zhong, Sheng Yang, and Guoyao Wu. "Free radicals, antioxidants, and nutrition." Nutrition 18.10 (2002): 872-879.‏

Apak, Reşat, et al. "Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report)." Pure and Applied Chemistry 85.5 (2013): 957-998.‏

Brand-Williams, Wendy, Marie-Elisabeth Cuvelier, and C. L. W. T. Berset. "Use of a free radical method to evaluate antioxidant activity." LWT-Food science and Technology 28.1 (1995): 25-30.‏

Fadda, Angela, et al. "Reaction time and DPPH concentration influence antioxidant activity and kinetic parameters of bioactive molecules and plant extracts in the reaction with the DPPH radical." Journal of Food Composition and Analysis 35.2 (2014): 112-119.‏

Bondet, V., W. Brand-Williams, and C. Berset. "Kinetics and mechanisms of antioxidant activity using the DPPH. free radical method." LWT-Food Science and Technology 30.6 (1997): 609-615.‏

[6] Li, Yan-feng, Pei-ze Li, and Zai-qun Liu. "Novel kinetic method for expressing the ability of antioxidant to scavenge radicals." Chemical Research in Chinese Universities 29.5 (2013): 947-951.‏

Volkov, V. A., N. A. Dorofeeva, and P. M. Pakhomov. "Kinetic method for studying the antiradical activity of medicinal plant extracts." Pharmaceutical chemistry journal 43.6 (2009): 333.‏

Haddadi, H., N. Alizadeh, and M. Shamsipur. "Stoichiometric and free radical-scavenging kinetic studies of extractable polyphenols from pomegranate husk and pistachio hull." Journal of the Iranian Chemical Society 8.3 (2011): 694-707.‏

Anissi, Jaouad, et al. "A comparative study of the antioxidant scavenging activity of green tea, black tea and coffee extracts: a kinetic approach." Food chemistry 150 (2014): 438-447.‏

Mimica-Dukic, N., and B. Bozin. "Mentha L. species (Lamiaceae) as promising sources of bioactive secondary metabolites." Current Pharmaceutical Design14.29 (2008): 3141-3150.‏

Kähkönen, Marja P., et al. "Antioxidant activity of plant extracts containing phenolic compounds." Journal of agricultural and food chemistry 47.10 (1999): 3954-3962.‏

Riachi, Liza G., and Carlos AB De Maria. "Peppermint antioxidants revisited." Food chemistry 176 (2015): 72-81.‏

Shahidi, Fereidoon, P. K. Janitha, and P. D. Wanasundara. "Phenolic antioxidants." Critical reviews in food science & nutrition 32.1 (1992): 67-103.‏

Osman, I. H. "In vitro antioxidant activity of Mentha pulegium from Saudi Arabia." Bioscience Research 10.1 (2013): 33-37.‏

Ahmed, Ahmed M., Hani A. Ozbak, and Hassan A. Hemeg. "Effect of essential oil of traditional two Saudi mint types and its possible role in cardiovascular and throat health." International journal of clinical and experimental medicine 8.5 (2015): 8060.‏

Zhuang, X. P., Y. Y. Lu, and G. S. Yang. "Extraction and determination of flavonoid in ginkgo." Chinese Herbal Medicine 23 (1992): 122-124.‏

Zhishen, Jia, Tang Mengcheng, and Wu Jianming. "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals." Food chemistry 64.4 (1999): 555-559.‏

Singleton, Vernon L., Rudolf Orthofer, and Rosa M. Lamuela-Raventós. "[14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent." Methods in enzymology 299 (1999): 152-178.‏

Sánchez‐Moreno, Concepción, Jose A. Larrauri, and Fulgencio Saura‐Calixto. "A procedure to measure the antiradical efficiency of polyphenols." Journal of the Science of Food and Agriculture 76.2 (1998): 270-276.‏

Gulluce, M., et al. "Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia." Food chemistry103.4 (2007): 1449-1456.‏

Scherer, Rodrigo, et al. "Antioxidant and antibacterial activities and composition of Brazilian spearmint (Mentha spicata L.)." Industrial crops and products 50 (2013): 408-413.‏

Özgen, U., et al. "Antioxidant properties of some medicinal Lamiaceae (Labiatae) species." Pharmaceutical biology 44.2 (2006): 107-112.‏

Scherer, Rodrigo, and Helena Teixeira Godoy. "Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method." Food chemistry 112.3 (2009): 654-658.‏

Espín, Juan Carlos, et al. "Anthocyanin-based natural colorants: a new source of antiradical activity for foodstuff." Journal of Agricultural and Food Chemistry48.5 (2000): 1588-1592.‏

Suja, Kizhiyedathu Polachira, Anathasankaran Jayalekshmy, and Chami Arumughan. "Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH• system." Journal of Agricultural and Food Chemistry 52.4 (2004): 912-915.‏

Brahmi, Fatiha, et al. "Chemical Composition and Biological Activities of Mentha Species." Aromatic and Medicinal Plants-Back to Nature. InTech, 2017.‏

National Toxicology Program. "Toxicology and carcinogenesis studies of pulegone (CAS No. 89-82-7) in F344/N rats and B6C3F1 mice (gavage studies)." National Toxicology Program technical report series 563 (2011): 1.‏

Savatović, Slađana M., et al. "Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts." Journal of the Serbian Chemical Society77.10 (2012): 1381-1389.‏

Al-Jaber, Nabilah A., Amani S. Awaad, and John E. Moses. "Review on some antioxidant plants growing in Arab world." Journal of Saudi Chemical Society15.4 (2011): 293-307.‏

Justesen, Ulla, and Pia Knuthsen. "Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes." Food chemistry 73.2 (2001): 245-250.‏

Riahi, Leila, et al. "Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia." Industrial crops and products49 (2013): 883-889.

Fialová, S., D. Tekeľová, and D. Grančai. "The content of phenolic compounds in underground and aerial parts of different Mentha species." Acta Facultatis Pharmaceuticae Universitatis Comenianae 59.1 (2012): 30-38.‏

Hajlaoui, Hafedh, et al. "Biological activities of the essential oils and methanol extract of tow cultivated mint species (Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine." World Journal of Microbiology and Biotechnology 25.12 (2009): 2227-2238.‏



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